Brescia University is located in Owensboro, Kentucky, near the Ohio River. The University campus is within walking distance of the revitalized downtown area, the RiverPark Performing Arts Center, the Daviess County Public Library and the Owensboro Museum of Fine Arts, as well as numerous restaurants, churches and parks. Many Owensboro industries and professional organizations partner with Brescia to provide enriching off-campus learning opportunities for students, particularly in the areas of business, education, social work, the arts, speech and hearing, and special education.

Owensboro is the industrial and cultural hub of western Kentucky. Owensboro is the third largest city in the Commonwealth of Kentucky, with a population of approximately 88,000. Owensboro is located 32 miles southeast of Evansville, Indiana; 123 miles north of Nashville, Tennessee; 109 miles southwest of Louisville, Kentucky; 203 miles southeast of St. Louis, Missouri; and 205 miles southwest of Cincinnati, Ohio.

Owensboro is known as "Kentucky's Festival City." Each year, the world-famous International Bar-B-Q Festival and the Summer Festival consists of spectators and participants from across the country and around the world.